ChemIONS

I carried out the experiment to see the effect of inhibitor folic acid on the action of catalase on hyd. perox i found that as i increased the concentration of folic acid the amount of oxygen produced increased rather than decreased as i had expected it to. I used extract from turmeric root as my enzyme. Can anyone suggest a second reaction that may have been going on simultaneously, that may have cause the increase in oxygen production. I am slkightly baffled? ......i recieved this reply....... I would have to ask how you were measuring the oxygen produced. Was is as oxygen, or just "gas" produced? If the latter, it might be possible that your tumeric root extract contains an decarboxylase enzyme, which might decarboxylate the folic acid, thereby producing CO2 gas. If you are measuring O2 directly as O2, then I too would be baffled...... i think this may be the case - im not really that glue'd up - but the guy who relied has a phd in biochemistry... so im asking 2 things. firstly - do you think he may be right>? and secondly - can u propose a way i which i could do the experiment again and actually measure the 02 specifically...